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Blast Program, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Participants, their background and applied data processing and important comments from their summaries.

Journal: Frontiers in Microbiology

Article Title: Proficiency Testing of Metagenomics-Based Detection of Food-Borne Pathogens Using a Complex Artificial Sequencing Dataset

doi: 10.3389/fmicb.2020.575377

Figure Lengend Snippet: Participants, their background and applied data processing and important comments from their summaries.

Article Snippet: P11 , Veterinary , – Host sequences were removed by blasting (BLASTn) against a database created from the Oncorhynchus mykiss isolate Swanson WGS data (NCBI acc. MSJN00000000.1) using an E-value cutoff 1E-100 – Taxonomic classification w/carried out by blasting (BLASTn) the remaining reads against an NCBI nt database using TimeLogic ® DeCypher ® server (Active Motif Inc., Carlsbad, CA, United States) with an E-value cutoff of 1E-5. The assignment of sequences to species were carried out by an in-house Python script using the nucl_gb.accessions2taxid (accession to taxid) and names.dmp (taxid to scientific names) files available from the resources at NCBI , – We included Murine norovirus in the table despite it is not human pathogen.

Techniques: Virus, Transmission Assay, Sequencing, Control, Cloning, Plasmid Preparation